Mechanism of trifluoromethionine resistance in Entamoeba histolytica

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Mechanism of trifluoromethionine resistance in Entamoeba histolytica.

OBJECTIVES To determine the mechanism of trifluoromethionine resistance in Entamoeba histolytica and evaluate the impact of acquired drug resistance on virulence. METHODS Trifluoromethionine-resistant amoebae were selected in vitro and examined for cross-resistance to antiamoebic drugs, stability of resistance, methionine γ-lyase (MGL) activity, cell adhesion and virulence. Targeted gene sile...

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Transcriptional and functional analysis of trifluoromethionine resistance in Entamoeba histolytica.

OBJECTIVES Drug resistance in parasitic protozoa is an obstacle to successful chemotherapy. Understanding how pathogens respond to drugs is crucial in preventing resistance. Previously, we have shown that in Entamoeba histolytica, methionine γ-lyase (EhMGL) downregulation results in trifluoromethionine resistance. The transcriptional response, however, of this parasite to the drug is not known....

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ژورنال

عنوان ژورنال: Journal of Antimicrobial Chemotherapy

سال: 2011

ISSN: 0305-7453,1460-2091

DOI: 10.1093/jac/dkr238